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diff quik staining method  (Sysmex Corporation)

 
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    Sysmex Corporation diff quik staining method
    Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics <t>using</t> <t>Diff-Quik</t> staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.
    Diff Quik Staining Method, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diff quik staining method/product/Sysmex Corporation
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    1) Product Images from "Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis"

    Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis

    Journal: Clinical and Experimental Otorhinolaryngology

    doi: 10.21053/ceo.2025-00170

    Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.
    Figure Legend Snippet: Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

    Techniques Used: Diff-Quik, Staining, Standard Deviation



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    Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

    Journal: Clinical and Experimental Otorhinolaryngology

    Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis

    doi: 10.21053/ceo.2025-00170

    Figure Lengend Snippet: Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

    Article Snippet: Slides were stained using the Diff-Quik staining method (Sysmex).

    Techniques: Diff-Quik, Staining, Standard Deviation